磷酸二脂酶
中文名稱：磷酸二脂酶
其他中文名稱：磷酸二酯酶Ⅱ；磷酸二酯酶
英文名稱： Phosphodiesterase II from bovine spleen
其他英文名稱： 3′-Exonuclease；Spleen exonuclease；Spleen phosphodiesterase
產(chǎn)品規(guī)格： BR，10u/mg
包裝： 10U
CAS號：9068-54-6
級別：BR
Activity：≥10u/mg
磷酸二脂酶活力定義：One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate
產(chǎn)品描述：Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique
性狀：粉末
用途：生化研究。
磷酸二脂酶保存：-20℃