商品描述:
商品屬性
商品描述:
商品屬性
GST瓊脂糖凝膠4FF
中文名稱: GST瓊脂糖凝膠4FF
其他中文名稱: 谷胱甘肽瓊脂糖凝膠4FF
英文名稱: Glutathione Sepharose 4FF
產(chǎn)品規(guī)格: 進分
包裝:5毫升
產(chǎn)品規(guī)格: 國產(chǎn)
包裝:10毫升
級別:BR
GST瓊脂糖凝膠4FFLigand:glutathione and 10-carbon linker arm
Ligand concentration:120-320umol glutathione/ml medium
Binding capacity:~10mg recombinant glutathione S-transferase/ml medium,GST Mr26000
Dynamic binding capacity:~11mg GST-tagged protein/ml medium Mr43000(GSTrap FF 1ml at 1 ml/min=156cm/h)
Mean particle size:90um
Bead structure:highly cross-linked 4% agarose
Maximum flow rate:450cm/h(15ml/min),using xk16/20 column with 5cm bed height,run at room temperature with aqueous buffer
Recommended flow rate:Sample loading:<100cm/h(<3ml/min using XK16/20 column);Washing and elution:100~300ml/min using XK16/20 column)
PH stability:3-12
Chemical stability:All commonly used aqueous buffers,e.g. 1M acetate pH 4.0 and 6M guanidine hydrochloride for 1 hour at room temperature
配基:谷胱甘肽
基質:6%的交聯(lián)瓊脂糖凝膠
配基密度:>40μm/ml
載量:2.1-2.7 mg GST 融合蛋白/ml gel
介質的顆粒大?。?0-160μm
最大流速:200cm/h
pH 范圍:6~9
化學穩(wěn)定性:所有常用的緩沖液
性狀:含20%乙醇,底部為乳白色膠體
GST瓊脂糖凝膠4FF用途:生化研究。專門用于純谷胱甘肽S-轉移酶(GST)融合蛋白、其他的谷胱甘肽轉移酶以及與谷胱甘肽有親和作用的親和色譜填料,一步分離就可得到很純的目標蛋白,純化條件溫和,可保證蛋白的活性
保存:2~8℃